Helium-Neon Laser Effects on Human Whole Blood by Spectroscopy In vitro Study
Mohammed Ali Haimid *
Department of Physics, Faculty of Pure and Applied Science, International University of Africa, Sudan.
Ali A. S. Marouf
Institute of Laser, Sudan University of Science and Technology, Sudan.
M. D. Abdalla
Department of Physics, Faculty of Science, Sudan University of Science and Technology, Sudan.
*Author to whom correspondence should be addressed.
Abstract
Low-power helium-neon laser recently has been used numerously in medical applications. FTIR and UV-Vis spectroscopic technique is employed to study the spectral differences in the serum of whole blood samples.
Aims: To study (He-Ne) laser (λ= 632 nm, power=2 mW) effect on human whole blood, after irradiated to different times from 10 min to 50 min.
Study Design: Human Whole Blood Irradiated to (He-Ne) laser (λ= 632 nm, power=2 mW).
Place and Duration of Study: Institute of Laser, Sudan University of science and technology (SUST), Soba Hospital, Khartoum- Sudan, February 2018.
Methodology: Blood samples were collected from healthy volunteers; blood sample exposed to (H-N) laser and control compared; UV-Vis spectrophotometer and FTIR were used to study the effect of laser radiation.
Results: Absorption spectrum and FTIR spectra of whole blood are compared before and after He-Ne laser radiation shows, a significant decrease in intensity. FTIR spectrum of non exposed blood showed the peaks due to O-H (free group), C=O (amide I group), N=O (nitro group), and C-H (aromatic group). N-H (Amino acid (amide II) Laser radiation changes in transmittance in FTIR spectra for C=O group and O-H, N=O, the percentage of transmittance were increased. The most effects are found when whole blood irradiated to He-Ne laser radiation for 10 and 20 min and transmittance decreases for C-H, and N-H, due to denaturation of the protein.
Conclusion: Photodegradation of blood components due to absorption of laser radiation causes changes in the structure and conformational changes in the polypeptide and decrease intensity.
Keywords: Laser, blood, UV-Vis, FTIR, spectroscopic