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Spectroscopic techniques can advantageously be employed for qualitative and quantitative analysis of plant isolates. In the present work, we dealt with the nature of visible absorption spectra of hexane and methanolic isolates of a medicinal plant S. monostachyus. S. monostachyus is a plant known to be rich in phytoconstituents that are regarded as compounds possessing beneficial biological activities and, in view of these phenomena, an attempt at identifying such phytoconstituents present in S. monostachyus was undertaken.
Spectroscopic data of methanolic isolate gave UV max at 215 nm (Emax 21000) with an average sharp peak indicating the chromophore –C=C- (K-band, Emax > 10000). There was a major I.R band of 2955.10 cm-1 (C-H stretch) of alkyl group among others. The GC-MS detected Octacosane (RT: 27.651, 13.25%), large molecular weight hydrocarbon and hexadecane (RT: 25.557, 13.00%) while the hexane isolate gave UV max at 224 nm (Emax 13000) with an average sharp peak indicating the chromophore –COO- (K band, Emax > 10000). Major I.R bands were 2919.14 cm-1 (C-H stretch), 1741.21 cm-1 (C=O) among others. The GC-MS detected Hexadecanoic acid (RT: 16.608, 40.16%), a large molecular fatty acid and Oleic acid (RT: 19.017, 21.54%).