Phytochemical Screening, Antioxidant and Antimicrobial Activities of the Essential Oils and Ethanol Extract of Psidium guajava Leaf

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Andrew Emmanuel
Dimas Kubmarawa
Galo Yahaya Sara
Akurenda Wahu


Objective: To investigate the Phytochemical screening, antioxidant and antimicrobial activities of the essential oil and ethanol extract of Psidium guajava.

Methods: The leaf of Psidium guajavabelongs to the myrtle family (Myrtacease) which is used as herbal remedies for the cure of many ailments by natives in northern part of Nigeria, was collected in June, 2018 from the Professor’s Quarters of Modibbo Adama University of Technology (MAUTECH) Yola. The leaf was air dried, pulverized and extracted by simple overnight maceration technique and then analyzed. Fresh leaf of the aforementioned was extracted using modified steam distillation. The phytochemical screening of the ethanol extract was carried out using standard method.

Results: The result revealed the present of Tannin, Flavanoid, Alkaloid, Volatile oil, Triterpene, Saponin, Glycoside while phenolic compound was absent in the ethanol extract of Psidium guajava. The result of the antioxidant activity of the essential oil was screened using DPPH method and the IC25 values of ascorbic acid (standard drug)  was 57.92 µl/m and Psidium guajava of the essential oil  was 46.55 µl/ml  respectively. Antibacterial activity was carried out using dics diffusion method and the results showed reasonable zone of inhibition against tasted organisms, with Staphylococcus epidemidis being the most inhibited (23 mm) and Proteus vulgari being the least inhibited (2 mm) with the ethanol extract of Psidium guajava. In contrast, Staphylococcus aureus was the most inhibited (13 mm) and Salmonella typhi showed the least inhibition (9mm) in the essential oil of Psidium guajava.

Conclusion: The result, thus support the use of the plants traditionally to treat chronic diarrhea, fever, diabetes, malaria and suggest its usage in the formulation of new antioxidant and antibacterial drugs.

Phytochemical screening, antioxidant, antimicrobial and Psidium guajava.

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How to Cite
Emmanuel, A., Kubmarawa, D., Sara, G. Y., & Wahu, A. (2019). Phytochemical Screening, Antioxidant and Antimicrobial Activities of the Essential Oils and Ethanol Extract of Psidium guajava Leaf. Asian Journal of Physical and Chemical Sciences, 7(4), 1-8.
Original Research Article


Nostro A, Germanò MP, D'angelo V, Marino A, Cannatelli MA. Extractionmethods and bioautography for evaluation of medicinal plant antimicrobial activity. Lett Appl Microbiol. 2000;30:379-384.

Rates SMK. Plants as source of drugs, Toxicon. 2001;39:603–613.

Food and Agriculture Organization. Trade in medicinal plants. In: Economic and Social Department, Food and Agriculture Organization of the United Nations, Rome. 2004;2–3.

Krishnaiah D, Sarbatly R, Bono A. Phytochemical antioxidants for health and medicine: A move towards nature. Biotechnol Mol Biol Rev. 2007;1:97-104.

Bashir ZA. In-Vitro Antimicrobial Activity of Membrane-Acting Antibiotics Action against Streptococci’. Journal of Applied Pharmaceutical Sciences. 2012;2(12): 042– 047.

Namukobea J, Kaseneneb JM, Kiremere BT, Byamukama R, Kamatenesi-Mugisha M, Krief S, Dumontet V, John D, Kabasa JD. Traditional plants used for medicinal purpose by local communities around northern sector of Kibole National park, Uganda. Journal of Ethnopharmacol. 2011;136:236–255.

Manikandan S, Ganesapanian S, Singh SM, Kumar agur AMK. “Emerging of multiple drug resistance human pathogens from urinary tract infections”. Curr. Res. Bacteriology. 2011;4:9–15.

World Health Organization. Drug Information. Herbal Medicine. 2000;14(4): 237-243.

Shears P. Antimicrobial resistance in tropics. Tropical Doctor. 2000;30(2):114 116.

Abebe D. The Development of Drug Research. Ethiopian Health and Nutrition Research Institution Newsletter.1996;1:5-6.

Irvine FR, Woody plants of Ghana. London: Oxford University Press; 1961.

Ticzon, Ticzon herbal medicine encyclopedia. Phillpines: Romeo R. Ticzon Company Publishing; 1997.

Abdelrahim SI, Almagboul AZ, Omer MEA, Elegami A. Antimicrobial activity of Psidium guajava L. Fitoterapia. 2002;73: 713-715.

Kumar M. A study of antibacterial activity of Psidium guajava Linn. Fruit extracts against gram-positive and gram-negative bacteria. International Journal of Institutional Pharmacy and Life Sciences. 2015;5(2):231-239.

Pavia DL, Lampman GM, Kriz GS. Engel RG. Introduction to Organic Laboratory Techniques - Small-Scale Approach, 1st Ed.; Harcourt Brace, Florida. 1998;165- 172.

Sofowora EA. Medicinal plants and traditional medicine in Africa, spectrum books limited, Ibadan, Nigeria. Text book chapter 1 and 2; 1993.

Mitscher LA, Leu RP,Balhala MS, Beal JI, White R. Antimicrobial agents from higher plants. Introduction, rational and methodology, Liayadia. 1972;35:157-66.

Ali NAA, Julich WD, Kusnick C, Lindequist U. Screening of Yemeni medicinal plants foranti bacterial and cytotoxicactivities. Jethnophamacol. 2001:74:173-9.

Ogunwade IA. Composition patterns of the essential oils of the leaves of Eucalyptus, Thuja, Callitris & Melaleuca species growing in Nigeria. PhD. Thesis Department of Chemistry University of Nigeria; 2001.

Braudwilliams, Cuvelier, Berset. Use of free radical method to evaluate antioxidant activity. Food Microbiol. 1995; 18:261–268.

Kubmarawa D, Kidah MI, Shagal MH. Antimicrobial Activities of Essential oils fromsome medicinal and Aromatic plants. British Biotechnology Journal. 2016;14(3): 1-6.

Yahaya G, Faten A, Fred W, Hany A. Phytochemical screening, anti-oxidant activity and in vitro anticancer potential of ethanolic and water leaves extracts of Annona muricata (Graviola)., Asian Pacific Journal of Tropical Biomedicine; 2014.

Shagal MH, Kubmarawa D, Alim H. Preliminary phytochemical investigation and antimicrobial evaluation of roots, stem-bark and leaves extracts of Diospyros mespiliformis. International Research Journal of Biochemistry and Bioinformatics. 2012;2(1):011-015

Tschesche R. Advances in the chemistry of anti-biotres substances from higher plants; pharmacognosy international congress. Heidelberg New York: Verlog, Berlin; 1971.

Iwu MM, Plant flavonoids in biology and medicine, In Proceeding of 4th Annual Conference of the Nigeria Society for Pharmacology. University of Nigeria Nsukka; 1984.

Ogundaini AO. From greens into medicine taking a lead from nature InauguralLecture Series No.176. Nigeria: O.A.U Press Ltd, Ile-Ife. 2005;16:17-38.

Usman H, Abdulrahman FI, Ladan AA. Phytochemical and antimicrobial evaluation of Tribulus terrestris. L Zygophylaceae) growing in Nigeria Res, J. of BIOSC. Medwel J. 2007;2:244- 247.

Abok A, Adeyemi A, Jegede IA. Spectrophtometric estimation of anthraquinone content and antimicrobial potential of extracts of some cassia species used in herbal medicine in Ibadan Nigeria, Sci. Forum. 2000;3:57-63.

Baser KH. Hand Book of Essential Oil: Science, Technology, and Application. CRC Press, London. 2010;17-19.

Odabasoglu F, Aslan A, Cakir A. Comparison of antioxidant activity and phenolic content of three lichen species. Phytother Res. 2004;18:938-941.

Huang WY, Cai YZ, Zhang Y. Natural phenolic compounds from medicinal herbs and dietary plants: Potential use for cancer prevention. Nutr Cancer. 2010;62(1):1-20. DOI:10.1080/01635580903191585.

Rice-Evans C, Miller N, Paganga G. Antioxidant properties of phenolic compounds. Trends in Plant Science. 1997;2:152-159.

Scalbert A. Antimicrobial properties of tannins. Phytochemistry. 1991;30:3875- 3883.