Analysis of Movements in Spinal Cord Hemisection Treatment with Amniotic Membrane – Preclinical Study
Asian Journal of Physical and Chemical Sciences,
Aims: This study aimed to evaluate the efficacy of Amniotic Membrane application in rats with Spinal Cord Injury induced by transverse hemisection using kinematic analysis and Sciatic Functional Index.
Study design: True experimental research design.
Place and Duration of Study: Instiuto de Pesquisa e Desenvolvimento (IP&D) of Universidade do Vale do Paraíba (UNIVAP), between September 2016 and December 2017.
Methodology: Fifteen adult male rats were used, allocated into three equal groups: Control (the spinal cord injury and Amniotic Membrane application were simulated), Lesion (spinal cord injury not treated), Amniotic membrane (spinal cord injury treated by amniotic membrane). All animals underwent surgical procedures. A transverse hemisection was performed in groups Lesion and Amniotic Membrene. A fragment of the biomaterial was applied in group AM covering the hemisection area.
Results: Sciatic Functional Index and motion analysis were performed by comparing images taken at pre- and postoperative time at 7, 14, 21, and 28 days. The kinematic analysis showed a significant difference between groups Control and Lesion at 7 days (p = 0.023) and 14 days (p = 0.015), and between groups Lesion and Amniotic Membrane at 14 days (p = 0.039), comparing the postoperative periods. The Sciatic Functional Index revealed significant differences between Groups Control and Lesion at 7 (p = -0.002), 14 (p = 0.003), and 21 days (p = 0.009), between Groups Control and Amniotic Membrane at 7 (p = 0.014), 14 (p = 0.007), and 28 days (p = 0; 013), and between Groups Lesion and Amniotic Membrane only at 14 days (p = 0.039).
Conclusion: Application of amniotic membrane in spinal cord hemisection in rats induced gait recovery and improvement in SFI compared to the untreated group.
- Spinal cord injury
- amniotic membrane
- kinematic analysis
- sciatic functional index
How to Cite
Zhou J, et al. Identification of the spinal expression profile of non-coding RNAs involved in neuropathic pain following spared nerve injury by sequence analysis. Frontiers in Molecular Neuroscience. 2017;10:91.
Winter B, Pattani H, Temple E. Spinal cord injury. Anaesthesia & Intensive Care Medicine. 2017;18(8): 404-409.
Kumar R, et al. Traumatic spinal injury: global epidemiology and worldwide volume. World Neurosurgery. 2018;113:e345-e363.
Ibrahim E, Lynne CM, Brackett NL. Male fertility following spinal cord injury: an update. Andrology. 2016;4(1):13-26.
James ND, et al. Neuromodulation in the restoration of function after spinal cord injury. The Lancet Neurology. 2018;17(10):905-917.
Xu Z, et al. Resident Microglia Activate before Peripheral Monocyte Infiltration and p75NTR Blockade Reduces Microglial Activation and Early Brain Injury after Subarachnoid Hemorrhage. ACS Chemical Neuroscience. 2018;10(1): 412-423.
Zeman RJ, et al. Stereotactic radiosurgery improves locomotor recovery after spinal cord injury in rats. Neurosurgery. 2008;63(5):981-988.
Wang Y, et al. Melatonin attenuates pain hypersensitivity and decreases astrocyte-mediated spinal neuroinflammation in a rat model of oxaliplatin-induced pain. Inflammation. 2017;40(6):2052-2061.
Paula AA, et al. Low-intensity laser therapy effect on the recovery of traumatic spinal cord injury. Lasers in Medical Science. 2014;29(6):1849-1859.
Arisawa EAL, et al. Amniotic Membrane in the Treatment of Spinal Cord Injuries. Injury. 2017;161:217-233.
Manuelpillai U, et al. Amniotic membrane and amniotic cells: potential therapeutic tools to combat tissue inflammation and fibrosis? Placenta. 2011;32: S320-S325.
Starecki M, Schwartz JA, Grande DA. Evaluation of amniotic-derived membrane biomaterial as an adjunct for repair of critical sized bone defects. Advances In Orthopedic Surgery; 2014.
Ullah I, Subbarao RB, Rho GJ. Human mesenchymal stem cells-current trends and future prospective. Bioscience Reports. 2015;35(2):e00191.
Sant'anna LB, et al. Amniotic membrane application reduces liver fibrosis in a bile duct ligation rat model. Cell Transplantation. 2011;20(3):441-453.
Loukogeorgakis SP, De Coppi P. Concise review: amniotic fluid stem cells: the known, the unknown, and potential regenerative medicine applications. Stem Cells. 2017;35(7):1663-1673.
Shoaib KK, Shakoor T, Amin MS. Presentation and Surgical Management of Epibulbar (Limbal) Dermoids. Pakistan Journal of Ophthalmology. 2018;34(3): 1663-1673.
Nicodemo MC, et al. Amniotic membrane as an option for treatment of acute Achilles tendon injury in rats. Acta Cirurgica Brasileira. 2017;32(2):125-139.
Oliveira EF, Mazzer N, Barbieri CH, Selli M. Correlation between functional index and morphometry to evaluate recovery of the rat sciatic nerve following crush injury: experimental study. J Reconstr Microsurg. 2011;17(1):69–75.
Varejão ASP, Meek MF, Ferreira AJA, Patrício JAB, Cabrita AMS. Functional evaluation of peripheral nerve regeneration in the rat: walking track analysis. J Neurosci Methods. 2001;108:1–9.
Paula AA, et al. Low-intensity laser therapy effect on the recovery of traumatic spinal cord injury. Lasers In Medical Science. 2014;29(6):1849-1859.
Nas K, et al. Rehabilitation of spinal cord injuries. World journal of orthopedics. 2015;6(1):8.
Ahuja CS, et al. Traumatic spinal cord injury. Nature Reviews Disease Primers. 2017;3(1):1-21.
Wang M, et al. Bioengineered scaffolds for spinal cord repair. Tissue Engineering Part B: Reviews. 2011;17(3):177-194.
Rouanet C, et al. Traumatic spinal cord injury: current concepts and treatment update. Arquivos de Neuro-Psiquiatria. 2017;75(6):387-393.
Yoon SY, et al. Spinal astrocyte gap junctions contribute to oxaliplatin-induced mechanical hypersensitivity. The Journal of Pain. 2013;14(2):205-214.
Kim SJ, et al. Anti-inflammatory effect of tauroursodeoxycholic acid in RAW 264.7 macrophages, bone marrow-derived macrophages, BV2 microglial cells, and spinal cord injury. Scientific Reports. 2018;8(1):1-11.
Navas A, et al. Anti‐inflammatory and anti‐fibrotic effects of human amniotic membrane mesenchymal stem cells and their potential in corneal repair. Stem Cells Translational Medicine. 2018;7(12):906-917.
Goel A. Stem cell therapy in spinal cord injury: Hollow promise or promising science? Journal of Craniovertebral Junction & Spine. 2016;7(2):121.
Jirsova K, Jones GLA. Amniotic membrane in ophthalmology: properties, preparation, storage and indications for grafting—a review. Cell and Tissue Banking. 2017;18(2):193-204.
Sant'anna LB, Hage R, Cardoso MAG, Arisawa EAL, Cruz MM, Parolini O, et al. Antifibrotic Effects of Human Amniotic Membrane Transplantation in Established Biliary Fibrosis Induced in Rats. Cell Transplant. 2016;25(12):2245-57.
Sane, MS, et al. Biochemical characterization of pure dehydrated binate amniotic membrane: role of cytokines in the spotlight. Regenerative Medicine. 2018;13(6):689-703.
Özbölük Ş, et al. The effects of human amniotic membrane and periosteal autograft on tendon healing: experimental study in rabbits. Journal of Hand Surgery (European Volume). 2010;35(4):262-268.
Shaw KA, et al. The Science and Clinical Applications of Placental Tissues in Spine Surgery. Global Spine Journal. 2018;8(6):629–637.
Zhou HL, Zhang XJ, Zhang MY, et al. Transplantation of Human Amniotic Mesenchymal Stem Cells Promotes Functional Recovery in a Rat Model of Traumatic Spinal Cord Injury. Neurochem Res. 2016;41:2708–2718.
Coutinho EST, et al. Raman spectroscopy of healthy, injured and amniotic membrane treated rat spinal cords. Spectrochimica Acta Part A: Molecular and Biomole- cular Spectroscopy. 2022;265: 120323.
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